CO2 incubator: CB 53 series / BINDER Inc.

CO2 incubator with additional process controls

The BINDER CB series CO2 incubator is the premium class among the CO2 incubators. It is suitable for all sensitive incubation applications and ensures optimal cell growth. A BINDER CO2 incubator of the CB series is up to even complex cultivation experiments or individual environments.

Advantages:

Hot air sterilization at 180 °C (356 °F)
Seamless, deep-drawn inner chamber made of stainless steel
Unique BINDER technology (patented air jacket system, controlled condensation, etc.)

Areas of application:

Bio Tissue Engineering
In vitro Fertilization (IVF)
Clinics / University Hospitals

Features	Customer benefits	Characteristics
Sterilization	Complete elimination of bacteria, spores, etc. Reliable sterilization of the atmosphere and all surfaces Minimal sterilization costs	180 °C (356 °F) hot air sterilization Standards compliance, meets all relevant standards Automatic sterilization process
Permadry™ humidity system	Uniform osmotic cell pressure Optimum cell growth even with microwell plates No Provides no sources of contamination.	Controlled humidification system Defined recondensation point Dry interior walls High humidity Rapid humidity recovery times Simple water exchange through water pan
APT.line™ heating technology	Optimal, uniform cell growth throughout the inner chamber	Preheating chamber with VENTAIR™ air jacket Homogeneous temperature distribution Rapid recovery after temperature drop Accurate temperature control
Gas distribution	Stable pH value	Gas mixing head with venturi effect Homogeneous CO2 distribution Fast effective aeration
Inner chamber concept	Easy cleaning Fully usable volume 53, 150, 210 l No Provides no sources of contamination.	Seamless, deep-drawn inner chamber Integrated shelf support system Burr-free stainless steel perforated sheets with tilt protection
CO2 measurement	Stable pH value even with frequent door opening Long-term stable system Low maintenance costs	Single-beam infrared sensor Rapid response time Measures CO2 in real-time Independent of gas and humidity
Operating costs	Minimal operating costs Time savings	Minimal work and material costs for sterilization

Electronically controlled APT.line™ preheating chamber assuring temperature accuracy and best cell growth
Temperature range from 5 °C (9 °F) above ambient temperature to 60 °C (140 °F)
MCS controller for temperature and CO2 concentration
User-friendly LCD screen
- Easy-to-read menu guide
- Integrated electronic chart recorder
- Variety of options for the graphic display of process parameters
- Real time clock
Standard-compliant hot air sterilization at 180 °C (356 °F) (DIN 58947)
VENTAIR™ Jacket System
Drift-free infrared CO2 measurement system
Patented Gas mixing nozzle
Permadry System - condensation-free double-pan humidification system, maintains dry interior walls
Seamless deep-drawn inner chamber made of stainless steel with integrated shelf support system (flanges)
Electronic self-diagnostic system for errors with visual and audible alarms, as well as relay potential changeover contact for central monitoring
Independent temperature safety device class 3.1 (DIN 12880) with optical and audible temperature alarm
Tightly-fitted inner glass door
RS 422 interface for communication software APT-COM™ DataControlSystem
2 perforated stainless steel shelves for CB 53 with standard equipement and O2 control
Units are stackable with stacking adapter
Door lock
BINDER test confirmation

	CB 53
	Exterior dimensions
	Width (mm/inch)	580 / 22.8
	Height (incl. feet) (mm/inch)	720 / 28.3
	Depth (plus 55 mm (2.2 inch) for door handle and connection) (mm/inch)	550 / 21.7
	Wall clearance side (mm/inch)	50 / 1.97
	Wall clearance rear (mm/inch)	100 / 3.94
	Number of doors (ea.)	1
	Inner glass door(s)	1

	Interior dimensions
	Width (mm/inch)	400 / 15.7
	Height (mm/inch)	400 / 15.7
	Depth (mm/inch)	330 / 13.0
	Interior volume (l/cu.ft.)	53 / 1.9
	Number of shelves (standard/max.)	2 / 3
	Dimensions shelves width x depth (mm/inch)	396 x 289 / 15.6 x 11.4
	Load per shelf (kg/lbs.)	10 / 22.0
	Permitted total load (kg/lbs.)	30 / 66.1
	Weight (empty) (kg/lbs.)	60 / 133

	Temperature data
	Temperature range from 5 °C (9 °F) above ambient temperature to (°C/°F)	60 / 140
	Temperature variation at 37 °C (± K)	0.3
	Temperature fluctuation at 37 °C (± K)	0.1
	Recovery time after door was opened for 30 sec. at 37 °C (98.6 °F) (min.) 1)	3

	Humidity data
	Humidity (±2% r.h.)	95

	CO2 data
	CO2 range (vol. % CO2)	0 - 20
	Setting accuracy (Vol.-% CO2)	0.1
	Recovery time after door was opened for 30 sec. 1)
	at 5.0 vol. % CO2 (min.)	6
	CO2 measurement	IR
	Connection hose nozzle for CO2 for hose with internal diameter (mm/inch)	6 / 0.24

	O2 data
	O2 range (vol. % O2)	0.2 - 95
	Setting accuracy (Vol.-% O2)	0.1
	Recovery time after door was opened for 30 sec. 1)
	at 1.0 vol. % O2 (min.)	25
	at 5.0 vol. % O2 (min.)	9
	O2 measurement	ZrO2
	Connection hose nozzle for O2 / N2 for hose with internal diameter (mm)	6 / 0.24

	Electrical data
	Voltage (± 10%) 50/60 Hz (V)	230
	Voltage (± 10%) 60 Hz (V)	115
	Nominal power (kW)	1.0
	Energy consumption at 37 °C (98.6 °F) (W) 2)	70

1) up to 98 % of the set value
2) These values can be used for dimensioning air condition systems.

All technical data are specified for units with standard equipment at an ambient temperature of 25 °C (77 °F) and a line voltage fluctuation of ±10%. The temperature data is determined in accordance to factory standard following DIN 12880, respecting the recommended wall clearances of 10% of the height, width and depth of the inner chamber. All figures are typical average values for series devices. We reserve the right to alter technical specifications at any time.

BINDER Gas Supply Service

The external tank changer makes automatic changeover to a second tank possible once the first tank is empty. It has acoustic and visual alarms and is equipped with a zero-voltage alarm output for extreme notification systems. It can be used for maximum two CO2 incubators and is suitable for CO2, O2 and N2 tanks.

Gas-tight, 4-way divided inner glass door

For stable climate conditions in the broad chamber. Minimal loss of humidity, heat, and CO2 when loading. Ensures short recovery times.

Control for variable O2 values

For hyper- or hypoxic culture conditions. The O2 or N2 gas supply can be controlled using an additional control loop as needed. A zirconium oxide sensor (ZrO2) is used for the measurement.

Silicone access ports

For introducing external measuring instruments into the chamber. The access ports have a diameter of 30 mm (1.2 inch) and can be sealed on both sides using a silicone plug. They can be positioned on the back, left, and right sides.

Stacking adapter

Used for stacking of two CB incubators which are thermally isolated from each other. The incubators can thus be sterilized with hot air independent of each other. Available as stacking adapters, stacking frames or base frames.

APT-COM™ DataControlSystem GLP Edition

Software for GLP-compliant control, programming and documentation. Allows networking of up to 30 devices or controllers. Meets FDA 21 CFR Part 11 requirements.

Calibration certificate & validation

BINDER can significantly reduce the workload in qualifying and validating devices. Nobody knows our devices as well and has as much experience in certifications as we do.

CB 53
Silicone access port with two silicone plugs, 30 mm (1.2 inch), back	O
Lockable controller keyboard	O
Gas tank connection set for CO2 consisting of a gas tank pressure regulator (max. pressure 10 bar) with connection parts and 5 m (16.4 ft.) hose	O
Gas tank connection set for O2 consisting of a gas tank pressure regulator (max. pressure 10 bar) with connection parts and 5 m (16.4 ft.) hose	O
Gas tank connection set for N2 consisting of a gas tank pressure regulator max. pressure 10 bar) with connection parts and 5 m (16.4 ft.) hose	O
BINDER Gas Supply Service external gas tank replacement for connecting 2 gas tanks, either CO2 or N2, with audible and visual alarms, as well as potential-free alarm output	O
Unit conversion from RS 422 to Ethernet interface	O
4 - 20 mA analog outputs for temperature and CO2 measurements (e.g. chart recorder connection) with 6-pin DIN socket (output not adjustable)	O
Switched LEMO inner chamber socket (coverable) and LEMO connector (max. current carrying capacity 230 V AC - 3 A) (protection class IP 65)	O
Independent Intelligent Fail Safe monitoring function. Unique safety function used to prevent unnoticed deviations of the CO2 concentration from the set value. Note: Not possible in conjunction with access ports or divided inner glass door	O
Calibration certificate for temperature and CO2 (temperature measurement in center / CO2 measurement performed using analyzed test gas at 37 °C (98.6 °F) and 5% CO2	O
Calibration certificate for optional O2 control, O2 measurement with analyzed test gas 1% O2	O
Temperature measurement acc. to DIN 12880 (27 measuring points) at 37 °C (98.6 °F) or at specified temperature with measuring protocol and certificate	O
Manual for Primary Human Cell Culture, in English	O
Perforated shelf, stainless steel	O
Shelf, stainless steel for devided gas-tight inner glass door	O
Base on casters	O
Stacking adapter for direct thermal decoupled stacking of two C 53 CO2 incubators	O

BINDER CB 53: CO2 incubator incubator for customized callus and IVF applications

The BINDER CO2 incubator - CB series